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Detailed staining conditions and order of the Opal™-TSA mIHC 7-marker panel for human FFPE tissue sections

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 7-marker panel for human FFPE tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Concentration Assay, Incubation

Detailed staining conditions and order of the Opal™-TSA mIHC 6-marker panel for formalin free zinc salt-fixed tissue sections

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Detailed staining conditions and order of the Opal™-TSA mIHC 6-marker panel for formalin free zinc salt-fixed tissue sections

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Staining, Incubation

Multiplex manual staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Multiplex automated staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a representative human lymph node FFPE tissue section using the 7-marker TLS panel Representative region of interest (ROI) showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 7-marker TLS panel on human prostate cancer FFPE tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 7-marker TLS panel on human prostate cancer FFPE tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human melanoma tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human melanoma tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Multiplex automated staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human prostate cancer tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex automated staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in human prostate cancer tissue sections using the 7-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 6-marker TLS panel on murine gastric cancer tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 6-marker TLS panel on murine gastric cancer tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Marker, Staining

Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in murine gastric cancer tissue sections using the 6-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at a 20× magnification, and scale bars indicate 100 μm.

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet: Multiplex manual staining of a Grade 2 immature TLS (upper panel) and a Grade 3 mature TLS (lower panel) in murine gastric cancer tissue sections using the 6-marker TLS panel Representative ROIs showing merge (on left) and single staining (on right): CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), PNAd (cyan), and DAPI (blue). Images were taken at a 20× magnification, and scale bars indicate 100 μm.

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Multiplex Assay, Staining, Marker

Journal: STAR Protocols

Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry

doi: 10.1016/j.xpro.2022.101961

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).

Techniques: Polymer, Plasmid Preparation, Recombinant, Saline, Software, Staining, Imaging

Images representing immunohistochemical staining of CD3, CD14, CD19, CD56 and IL-6 in HCl group (A) and HCl-HA group (B). Black arrows indicate stained inflammatory cells. It is observed that inflammatory cell infiltration is more common in stroma and smooth muscle.

Journal: International Brazilian Journal of Urology : official journal of the Brazilian Society of Urology

Article Title: Impact of intravesical hyaluronic acid treatment on bladder inflammation in interstitial cystitis rat model

doi: 10.1590/S1677-5538.IBJU.2017.0713

Figure Lengend Snippet: Images representing immunohistochemical staining of CD3, CD14, CD19, CD56 and IL-6 in HCl group (A) and HCl-HA group (B). Black arrows indicate stained inflammatory cells. It is observed that inflammatory cell infiltration is more common in stroma and smooth muscle.

Article Snippet: After removal of excess serum, sections were incubated overnight in a humidified chamber at 4°C with primary antibodies: CD3 rabbit polyclonal antibody (Bioss Biotechnology; bS-0765R) in a 1:500 dilution; CD 14 rabbit polyclonal antibody (Bioss Biotechnology; bs-1192R) in a 1:500 dilution; CD19 rabbit polyclonal antibody (Bioss Biotechnology; bs-4755R) in a 1:400 dilution; CD 56 rabbit polyclonal antibody (Bioss Biotechnology; bS-0805R) in a 1:500 dilution; and IL-6 rabbit polyclonal antibody (Bioss Biotechnology; bs-4540R) in a 1:500 dilution.

Techniques: Immunohistochemical staining, Staining